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Addgene

pmEGFP-C1-Nir3-LNS2
(Plasmid #220087)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 220087 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1-mEGFP-C1
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    PITPNM2
  • Alt name
    Nir3
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    855
  • Mutation
    amino acids 925-1209
  • Entrez Gene
    PITPNM2 (a.k.a. NIR-3, NIR3, RDGB2, RDGBA2)
  • Promoter CMV
  • Tag / Fusion Protein
    • mEGFP-GGGGSHM (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BmtI (not destroyed)
  • 3′ cloning site NotI (not destroyed)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Dr. Shujuan Gao, StonyBrook University, NY

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pmEGFP-C1-Nir3-LNS2 was a gift from Gerry Hammond (Addgene plasmid # 220087 ; http://n2t.net/addgene:220087 ; RRID:Addgene_220087)

  • For your References section:

    Nir1-LNS2 is a novel phosphatidic acid biosensor that reveals mechanisms of lipid production. Weckerly CC, Rahn TA, Ehrlich M, Wills RC, Pemberton JG, Airola MV, Hammond GRV. bioRxiv [Preprint]. 2024 Feb 28:2024.02.28.582557. doi: 10.1101/2024.02.28.582557. 10.1101/2024.02.28.582557 PubMed 38464273
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