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Addgene

pNESx2-EGFP-C1-PABDx2-Spo20
(Plasmid #220082)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 220082 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP-C1
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Spo20
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    246
  • Mutation
    amino acids 51-91, tandem dimer
  • Entrez Gene
    SPO20 (a.k.a. YMR017W, DBI9)
  • Promoter CMV
  • Tag / Fusion Protein
    • X. leavis map2k1.L(32-44)-GGSG-X. leavis map2k1.L(32-44)-PVAT-EGFP-SGLRSRA (N terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pNESx2-EGFP-C1-PABDx2-Spo20 was a gift from Gerry Hammond (Addgene plasmid # 220082 ; http://n2t.net/addgene:220082 ; RRID:Addgene_220082)

  • For your References section:

    Nir1-LNS2 is a novel phosphatidic acid biosensor that reveals mechanisms of lipid production. Weckerly CC, Rahn TA, Ehrlich M, Wills RC, Pemberton JG, Airola MV, Hammond GRV. bioRxiv [Preprint]. 2024 Feb 28:2024.02.28.582557. doi: 10.1101/2024.02.28.582557. 10.1101/2024.02.28.582557 PubMed 38464273
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