TagBFP2-C1-SACM1LdeltaTMD-Fis1tail
(Plasmid #220078)

• Purpose: SAC1 with the TMD deleted and replaced with the Fis1tail to replace the TMD and target the mitochondria instead of the ER with a Blue Fluorophore
• Depositing Lab: Gerry Hammond
• Publication: Doyle et al Contact (Thousand Oaks). 2024 Feb 7;7:25152564241229272. doi: 10.1177/25152564241229272. eCollection 2024 Jan-Dec.

Backbone

• Vector backbone: pTagBFP2-C1
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: SACM1l-Fis1tail
• Alt name: SAC1
• Species: H. sapiens (human)
• Mutation: deltaTMD
• Entrez Gene: SACM1L (a.k.a. SAC1)

Cloning Information

• Cloning method: Gibson Cloning

Resource Information

• Supplemental Documents:
  • TagBFP2-C1-Sac1deltaTMD-Fis1Tail.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
• Industry Terms:
  • Not Available to Industry

Depositor Comments

SACM1L gene used in this plasmid contains a missense mutation Y433F relative to the human genome reference sequence. However, the short Genetic Variations database (dbSNP) shows that this allele (rs1468542) represents approximately 60% of alleles present in the human population sampled to date. This allele can therefore be viewed as ‘wild-type’.