Skip to main content
Addgene

EGFP-1-SAC1deltaTMD-Fis1tail
(Plasmid #220077)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 220077 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pEGFP-C1
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SACM1l-Fis1tail
  • Alt name
    SAC1
  • Species
    H. sapiens (human)
  • Mutation
    deltaTMD
  • Entrez Gene
    SACM1L (a.k.a. SAC1)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

SACM1L gene used in this plasmid contains a missense mutation Y433F relative to the human genome reference sequence. However, the short Genetic Variations database (dbSNP) shows that this allele (rs1468542) represents approximately 60% of alleles present in the human population sampled to date. This allele can therefore be viewed as ‘wild-type’.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    EGFP-1-SAC1deltaTMD-Fis1tail was a gift from Gerry Hammond (Addgene plasmid # 220077 ; http://n2t.net/addgene:220077 ; RRID:Addgene_220077)

  • For your References section:

    Orthogonal Targeting of SAC1 to Mitochondria Implicates ORP2 as a Major Player in PM PI4P Turnover. Doyle CP, Rectenwald A, Timple L, Hammond GRV. Contact (Thousand Oaks). 2024 Feb 7;7:25152564241229272. doi: 10.1177/25152564241229272. eCollection 2024 Jan-Dec. 10.1177/25152564241229272 PubMed 38327560
Related items: