mCherry-C1-ORP5deltaTMD-FKBP
(Plasmid
#220073)
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Purposeexpresses ORP5 with the transmembrane domain deleted. The mCherry fluorophore and FKBP are attached to ORP5.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 220073 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepmCherry-C1
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameOSBPL5deltaTMD
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Alt nameORP5deltaTMD
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SpeciesH. sapiens (human)
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MutationdeltaTMD
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Entrez GeneOSBPL5 (a.k.a. OBPH1, ORP5)
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
mCherry-C1-ORP5deltaTMD-FKBP was a gift from Gerry Hammond (Addgene plasmid # 220073 ; http://n2t.net/addgene:220073 ; RRID:Addgene_220073) -
For your References section:
Orthogonal Targeting of SAC1 to Mitochondria Implicates ORP2 as a Major Player in PM PI4P Turnover. Doyle CP, Rectenwald A, Timple L, Hammond GRV. Contact (Thousand Oaks). 2024 Feb 7;7:25152564241229272. doi: 10.1177/25152564241229272. eCollection 2024 Jan-Dec. 10.1177/25152564241229272 PubMed 38327560