mCherry-C1-FKBPflex-OSBP-siRNAr
(Plasmid
#220066)
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PurposesiRNA resistant ORP5 attcahed with anFKBP and a mCherry fluorophore
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 220066 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepmCherry-C1
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameOSBPL5 siRNA resistant
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SpeciesH. sapiens (human)
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MutationsiRNA resistant
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Entrez GeneOSBPL5 (a.k.a. OBPH1, ORP5)
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
mCherry-C1-FKBPflex-OSBP-siRNAr was a gift from Gerry Hammond (Addgene plasmid # 220066 ; http://n2t.net/addgene:220066 ; RRID:Addgene_220066) -
For your References section:
OSBP is a Major Determinant of Golgi Phosphatidylinositol 4-Phosphate Homeostasis. Doyle CP, Timple L, Hammond GRV. Contact (Thousand Oaks). 2024 Feb 22;7:25152564241232196. doi: 10.1177/25152564241232196. eCollection 2024 Jan-Dec. 10.1177/25152564241232196 PubMed 38405037