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Addgene

pAN057
(Plasmid #220053)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 220053 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP-N1
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    fLuc-CFTR(W1282X, exons 22-27)
  • Alt name
    CFTR
  • Alt name
    firefly luciferase
  • Species
    H. sapiens (human)
  • Mutation
    c.3846G>A mutation
  • Entrez Gene
    CFTR (a.k.a. ABC35, ABCC7, CF, CFTR/MRP, MRP7, TNR-CFTR, dJ760C5.1)

Cloning Information

  • Cloning method Gibson Cloning

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAN057 was a gift from Blake Wiedenheft (Addgene plasmid # 220053 ; http://n2t.net/addgene:220053 ; RRID:Addgene_220053)

  • For your References section:

    Repair of CRISPR-guided RNA breaks enables site-specific RNA excision in human cells. Nemudraia A, Nemudryi A, Wiedenheft B. Science. 2024 Apr 25:eadk5518. doi: 10.1126/science.adk5518. 10.1126/science.adk5518 PubMed 38662916
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