pAN013
(Plasmid
#220050)
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PurposeReporter plasmid that expresses GFP and firefly luciferase linked with a viral 2A peptide. A stop codon is inserted at the 3'-end of the gfp gene, which permits GFP, but not luciferase expression.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 220050 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepEGFP-N1
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameGFP-stop-fLuc
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Alt nameppluGFP2-pest
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Alt namefirefly luciferase
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SpeciesSynthetic
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAN013 was a gift from Blake Wiedenheft (Addgene plasmid # 220050 ; http://n2t.net/addgene:220050 ; RRID:Addgene_220050) -
For your References section:
Repair of CRISPR-guided RNA breaks enables site-specific RNA excision in human cells. Nemudraia A, Nemudryi A, Wiedenheft B. Science. 2024 Apr 25:eadk5518. doi: 10.1126/science.adk5518. 10.1126/science.adk5518 PubMed 38662916
