pAN007
(Plasmid
#220048)
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PurposeReporter plasmid that encodes for the GFP fused to an N-terminal flag-HA epitope. A stop codon is inserted between the epitope tag and the gfp sequence to terminate GFP translation.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 220048 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepEGFP-N1
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameflag-HA-stop-GFP
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Alt nameppluGFP2-pest
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Alt namefirefly luciferase
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SpeciesSynthetic
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAN007 was a gift from Blake Wiedenheft (Addgene plasmid # 220048 ; http://n2t.net/addgene:220048 ; RRID:Addgene_220048) -
For your References section:
Repair of CRISPR-guided RNA breaks enables site-specific RNA excision in human cells. Nemudraia A, Nemudryi A, Wiedenheft B. Science. 2024 Apr 25:eadk5518. doi: 10.1126/science.adk5518. 10.1126/science.adk5518 PubMed 38662916
