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Addgene

pAN099
(Plasmid #220044)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 220044 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUC19
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    crRNA
  • gRNA/shRNA sequence
    Unspecified
  • Species
    Synthetic

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAN099 was a gift from Blake Wiedenheft (Addgene plasmid # 220044 ; http://n2t.net/addgene:220044 ; RRID:Addgene_220044)

  • For your References section:

    Repair of CRISPR-guided RNA breaks enables site-specific RNA excision in human cells. Nemudraia A, Nemudryi A, Wiedenheft B. Science. 2024 Apr 25:eadk5518. doi: 10.1126/science.adk5518. 10.1126/science.adk5518 PubMed 38662916
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