Skip to main content
Addgene

pOCC120-MBP-Ded1-monoGFP
(Plasmid #219983)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 219983 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pOCC120
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Ded1p-monoGFP
  • Species
    S. cerevisiae (budding yeast)
  • Entrez Gene
    DED1 (a.k.a. YOR204W, SPP81)
  • Tags / Fusion Proteins
    • MBP (N terminal on insert)
    • monoGFP-6xHis (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

MBP- and His-tag can be cleaved with 3C-Protease

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pOCC120-MBP-Ded1-monoGFP was a gift from Simon Alberti (Addgene plasmid # 219983 ; http://n2t.net/addgene:219983 ; RRID:Addgene_219983)
Related items: