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Addgene

pOCC22-MBP-eIF4E
(Plasmid #219978)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 219978 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pOCC22
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    eIF4E
  • Species
    S. cerevisiae (budding yeast)
  • Entrez Gene
    CDC33 (a.k.a. YOL139C, TIF45)
  • Tag / Fusion Protein
    • MBP (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

MBP can be cleaved with 3C-protease

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pOCC22-MBP-eIF4E was a gift from Simon Alberti (Addgene plasmid # 219978 ; http://n2t.net/addgene:219978 ; RRID:Addgene_219978)
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