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Addgene

pOCC102-MBP-eIF4G-459
(Plasmid #219975)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 219975 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pOCC102
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    eIF4G-459
  • Species
    S. cerevisiae (budding yeast)
  • Mutation
    459LL460 to 459AA460
  • Entrez Gene
    TIF4631 (a.k.a. YGR162W)
  • Tag / Fusion Protein
    • 6xHis-MBP (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

MBP can be cleaved with 3C-protease

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pOCC102-MBP-eIF4G-459 was a gift from Simon Alberti (Addgene plasmid # 219975 ; http://n2t.net/addgene:219975 ; RRID:Addgene_219975)
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