pOCC102-MBP-eIF4G-213
(Plasmid
#219974)
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PurposeeIF4G-213 protein purification
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 219974 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepOCC102
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Vector typeInsect Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameeIF4G-213
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SpeciesS. cerevisiae (budding yeast)
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Mutation213VKLR216 to 213AAAA216
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Entrez GeneTIF4631 (a.k.a. YGR162W)
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Tag
/ Fusion Protein
- 6xHis-MBP (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
MBP can be cleaved with 3C-protease
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pOCC102-MBP-eIF4G-213 was a gift from Simon Alberti (Addgene plasmid # 219974 ; http://n2t.net/addgene:219974 ; RRID:Addgene_219974)
