pRSET-MagLOV
(Plasmid #219957)

• Purpose: Bacterial expression of MagLOV, a magnetic-field-sensitive fluorescent protein engineered from AsLOV2
• Depositing Lab: Andrew York
• Publication: Frank Hayward et al (2023) doi: 10.5281/zenodo.8137174

Backbone

• Vector backbone: pRSETB
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 2828
• Total vector size (bp): 3263
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: MagLOV
• Species: A. sativa
• Insert Size (bp): 435
• Mutation: AsLOV2 C450P L496V Q513K G528K D540M
• Promoter: T7
• Tag / Fusion Protein:
  • 6xHis (N terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7 terminator

Resource Information

• Supplemental Documents:
  • pRSET-MagLOV.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Described in the article "Magnetic control of the brightness of fluorescent proteins": https://doi.org/10.5281/zenodo.8137174, https://andrewgyork.github.io/gfp_magnetofluorescence/#MagLOV_update.

Read more about this plasmid on the Addgene Blog: https://blog.addgene.org/magnetic-control-of-proteins.