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Addgene

pRSET-MagLOV
(Plasmid #219957)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 219957 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRSETB
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 2828
  • Total vector size (bp) 3263
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MagLOV
  • Species
    A. sativa
  • Insert Size (bp)
    435
  • Mutation
    AsLOV2 C450P L496V Q513K G528K D540M
  • Promoter T7
  • Tag / Fusion Protein
    • 6xHis (N terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Described in the article: "Magnetic control of the brightness of fluorescent proteins" https://doi.org/10.5281/zenodo.8137174, https://andrewgyork.github.io/gfp_magnetofluorescence/#MagLOV_update.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRSET-MagLOV was a gift from Andrew York (Addgene plasmid # 219957 ; http://n2t.net/addgene:219957 ; RRID:Addgene_219957)

  • For your References section:

    Magnetic control of the brightness of fluorescent proteins. Frank Hayward R, Rai A, Lazzari-Dean, JR, Lefebvre AEYT, York AG, Ingaramo M. (2023) doi: 10.5281/zenodo.8137174 10.5281/zenodo.8137174
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