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HiFi-nCas9-NG_P2A-mCherry
(Plasmid #219936)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 219936 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMV
  • Vector type
    Mammalian Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Sp-HiFi-nCas9(D10A)-NG_P2A-mCherry
  • Species
    H. sapiens (human), Synthetic
  • Mutation
    HiFi-nCas9(D10A/R691A)
  • Promoter CMV
  • Tags / Fusion Proteins
    • SV40 bpNLS (N terminal on insert)
    • SV40 bpNLS-P2A-mCherry (C terminal on insert)

Cloning Information

  • Cloning method Other

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

QTC399; could also be used for dual-nicking or other editing modalities

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    HiFi-nCas9-NG_P2A-mCherry was a gift from Alexis Komor (Addgene plasmid # 219936 ; http://n2t.net/addgene:219936 ; RRID:Addgene_219936)

  • For your References section:

    Development of multiplexed orthogonal base editor (MOBE) systems. Cowan QT, Gu S, Gu W, Ranzau BL, Simonson TS, Komor AC. Nat Biotechnol. 2024 May 21. doi: 10.1038/s41587-024-02240-0. 10.1038/s41587-024-02240-0 PubMed 38773305
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