nCas9-NG_P2A-mCherry
(Plasmid
#219934)
-
PurposeMammalian expression of Sp-nCas9-NG with P2A-mCherry marker for use with MOBE systems
-
Depositing Lab
-
Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 219934 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backbonepCMV
-
Vector typeMammalian Expression, CRISPR
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameSp-nCas9(D10A)-NG_P2A-mCherry
-
SpeciesH. sapiens (human), Synthetic
-
MutationnCas9(D10A)
- Promoter CMV
-
Tags
/ Fusion Proteins
- SV40 bpNLS (N terminal on insert)
- SV40 bpNLS-P2A-mCherry (C terminal on insert)
Cloning Information
- Cloning method Other
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
QTC24; could also be used for dual-nicking or other editing modalities
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
nCas9-NG_P2A-mCherry was a gift from Alexis Komor (Addgene plasmid # 219934 ; http://n2t.net/addgene:219934 ; RRID:Addgene_219934) -
For your References section:
Development of multiplexed orthogonal base editor (MOBE) systems. Cowan QT, Gu S, Gu W, Ranzau BL, Simonson TS, Komor AC. Nat Biotechnol. 2024 May 21. doi: 10.1038/s41587-024-02240-0. 10.1038/s41587-024-02240-0 PubMed 38773305
