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Addgene

pCfb13225
(Plasmid #219900)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 219900 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Our own backbone
  • Vector type
    Yeast Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Contains gRNA targeting TF35 (YALI1_E34586g) and homologous arm matching TF35
  • gRNA/shRNA sequence
    CGGTAGGGTACCCTTCGTCG
  • Species
    Yarrowia lipolytica

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This plasmid contains gRNA targeting TF35 (YALI1_E34586g) and homologous arm matching TF35, which can be used for library construction by inserting promoters using a golden gate or directly knocking TF35 out.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCfb13225 was a gift from Irina Borodina (Addgene plasmid # 219900 ; http://n2t.net/addgene:219900 ; RRID:Addgene_219900)
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