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Addgene

pCfb13222
(Plasmid #219897)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 219897 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Our own backbone
  • Vector type
    Yeast Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Contains gRNA targeting TF32 (YALI1_F33301g) and homologous arm matching TF32
  • gRNA/shRNA sequence
    GTGCAGTGTTTGGACTGCTG
  • Species
    Yarrowia lipolytica

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This plasmid contains gRNA targeting TF32 (YALI1_F33301g) and homologous arm matching TF32, which can be used for library construction by inserting promoters using a golden gate or directly knocking TF32 out.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCfb13222 was a gift from Irina Borodina (Addgene plasmid # 219897 ; http://n2t.net/addgene:219897 ; RRID:Addgene_219897)
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