PASTE payload acceptor with attP site pDY2087
(Plasmid
#219859)
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PurposeBxb1 payload vector with 5' attP
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Depositing Labs
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 219859 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepDY0181 Cargo EGFP with AttP Bxb1 site (Addgene 179115)
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Vector typeCRISPR
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameEGFP
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
PASTE payload acceptor with attP site pDY2087 was a gift from Omar Abudayyeh & Jonathan Gootenberg (Addgene plasmid # 219859 ; http://n2t.net/addgene:219859 ; RRID:Addgene_219859) -
For your References section:
Precise kilobase-scale genomic insertions in mammalian cells using PASTE. Fell CW, Schmitt-Ulms C, Tagliaferri DV, Gootenberg JS, Abudayyeh OO. Nat Protoc. 2024 Dec 15. doi: 10.1038/s41596-024-01090-z. 10.1038/s41596-024-01090-z PubMed 39676077