T7-RelA
(Plasmid #21984)

• Depositing Lab: Warner Greene
• Publication: Chen Lf et al Science. 2001 Aug 31. 293(5535):1653-7.

Backbone

• Vector backbone: pEv3s
• Backbone manufacturer: Matthias P et al. Nucleic Acids Research (1989). 17(15): 6418
• Backbone size w/o insert (bp): 5120
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: NFkB p65 subunit
• Alt name: RelA
• Species: H. sapiens (human)
• Insert Size (bp): 1600
• Mutation: For more details for this expression construct, as well as associated mutants, please contact: Dr. Lin-Feng Chen ()
• Entrez Gene: RELA (a.k.a. AIF3BL3, CMCU, NFKB3, p65)
• Tag / Fusion Protein:
  • T7 gp10 (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: NTer primer 5'-GGGGGATCTTGGTGGCGTG-3'
• 3′ sequencing primer: CTer primer: 5'-CCCTGAAAACTTTGCCCCCTCC-3'

Resource Information

• Articles Citing this Plasmid:
  • 8 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry