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Addgene

LncRNA overexpression EF1α_BbsI_SV40 polyA_EGFP
(Plasmid #219828)

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Item Catalog # Description Quantity Price (USD)
Plasmid 219828 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pUC19 (Addgene #50005)
  • Backbone size (bp) 2700
  • Modifications to backbone
    Addition of lncRNA expression cassette (EF1a, MluI, BbsI, SV40 polyA) Addition of eGFP
  • Vector type
    Mammalian Expression
  • Promoter EF1a
  • Selectable markers
    eGFP

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Cloning Information

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    LncRNA overexpression EF1α_BbsI_SV40 polyA_EGFP was a gift from Philipp Maass (Addgene plasmid # 219828 ; http://n2t.net/addgene:219828 ; RRID:Addgene_219828)
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