LncRNA overexpression EF1α-BbsI-SV40polyA_puroR
(Plasmid
#219826)
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Purpose(Empty Backbone) Overexpression vector for non-coding RNAs driven by EF1α promoter with puromycin resistance. Cloning using BbsI.
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 219826 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * | |
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Backbone
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Vector backbonepUC19 (Addgene #50005)
- Backbone size (bp) 2700
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Modifications to backboneAddition of lncRNA overexpression cassette (pEF1a, MluI, BbsI, SV40 polyA) Addition of puromycin resistance
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Vector typeMammalian Expression
- Promoter EF1a
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer EF1a forward (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
LncRNA overexpression EF1α-BbsI-SV40polyA_puroR was a gift from Philipp Maass (Addgene plasmid # 219826 ; http://n2t.net/addgene:219826 ; RRID:Addgene_219826)
