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Addgene

CMV-dCas9_puroR
(Plasmid #219825)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 219825 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEF_dCas9 (Addgene #68416)
  • Backbone size w/o insert (bp) 4600
  • Total vector size (bp) 8600
  • Modifications to backbone
    Changed EF1a promoter to CMV Addition of puromycin resistance
  • Vector type
    Mammalian Expression, CRISPR
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    dCas9
  • Species
    Streptococcus pyogenes
  • Insert Size (bp)
    4000
  • Mutation
    D10A/H841A
  • Promoter CMV

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CMV-dCas9_puroR was a gift from Philipp Maass (Addgene plasmid # 219825 ; http://n2t.net/addgene:219825 ; RRID:Addgene_219825)
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