dCas9-miniTurbo
(Plasmid
#219822)
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PurposeEF1α-driven expression of dCas9 fused to miniTurbo with GFP (Adapted from plasmid #153209)
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 219822 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneCARPID BASU-dCasRx (Addgene #153209)
- Backbone size w/o insert (bp) 10000
- Total vector size (bp) 14999
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Modifications to backboneAddition of dCas9:miniTurbo
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Vector typeMammalian Expression, CRISPR
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Selectable markersGFP
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namedCas9
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SpeciesStreptococcus pyogenes
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Insert Size (bp)4000
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MutationD10A/H841A
- Promoter EF1a
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Tag
/ Fusion Protein
- miniTurbo (C terminal on insert)
Cloning Information
- Cloning method Gene Synthesis
- 5′ sequencing primer EF1a (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
dCas9-miniTurbo was a gift from Philipp Maass (Addgene plasmid # 219822 ; http://n2t.net/addgene:219822 ; RRID:Addgene_219822)
