dCas9-miniTurbo
(Plasmid #219822)

• Purpose: EF1α-driven expression of dCas9 fused to miniTurbo with GFP (Adapted from plasmid #153209)
• Depositing Lab: Philipp Maass
• Publication: LncRNA CISTR-ACT regulates cell size in human and mouse (unpublished)

Backbone

• Vector backbone: CARPID BASU-dCasRx (Addgene #153209)
• Backbone size w/o insert (bp): 10000
• Total vector size (bp): 14999
• Modifications to backbone: Addition of dCas9:miniTurbo
• Vector type: Mammalian Expression, CRISPR
• Selectable markers: GFP

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: dCas9
• Species: Streptococcus pyogenes
• Insert Size (bp): 4000
• Mutation: D10A/H841A
• Promoter: EF1a
• Tag / Fusion Protein:
  • miniTurbo (C terminal on insert)

Cloning Information

• Cloning method: Gene Synthesis
• 5′ sequencing primer: EF1a

Resource Information

• Supplemental Documents:
  • dCas9-miniTurbo AddGene.gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
• Industry Terms:
  • Not Available to Industry