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Addgene

EGFP-hAgo2
(Plasmid #21981)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 21981 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    EGFP-C1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Argonaute 2
  • Alt name
    eiF2C2, Ago2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2580
  • Entrez Gene
    AGO2 (a.k.a. CASC7, EIF2C2, LESKRES, LINC00980, PPD, Q10)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (unknown if destroyed)
  • 3′ cloning site BamHI (unknown if destroyed)
  • 5′ sequencing primer n/a
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    EGFP-hAgo2 was a gift from Phil Sharp (Addgene plasmid # 21981 ; http://n2t.net/addgene:21981 ; RRID:Addgene_21981)
  • For your References section:

    Quantitative analysis of Argonaute protein reveals microRNA-dependent localization to stress granules. Leung AK, Calabrese JM, Sharp PA. Proc Natl Acad Sci U S A. 2006 Nov 28. 103(48):18125-30. 10.1073/pnas.0608845103 PubMed 17116888