Lp-BFPnls-LP-Cherry-T2A-NTR2.0
(Plasmid
#219785)
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PurposeUsed for generating a transgenic line for inducing NTR2.0 expression in Cre positive cells after conversion
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 219785 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneLp-NTR 2.0
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemTagBFP2
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SpeciesSynthetic
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Tag
/ Fusion Protein
- 3x SV40 NLS (C terminal on insert)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2025.03.30.645595 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Lp-BFPnls-LP-Cherry-T2A-NTR2.0 was a gift from Prayag Murawala (Addgene plasmid # 219785 ; http://n2t.net/addgene:219785 ; RRID:Addgene_219785) -
For your References section:
The essential role of connective-tissue cells during axolotl limb regeneration. García-García D, Knapp D, Kim M, Jamwal K, Fuqua H, Seaman RP, Grindle RE, Nowoshilow S, Novatchkova M, Kolling FW, Graber JH, Murawala P. bioRxiv 2025.03.30.645595; doi: 10.1101/2025.03.30.645595 10.1101/2025.03.30.645595