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Addgene

pAAV-CMV-CD4-sTC-M13-GFP-IRES-CaM-V5-sTN
(Plasmid #219782)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 219782 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    AAV
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    CD4-sTC-M13-GFP-IRES-CaM-V5-sTN
  • Species
    Synthetic
  • Promoter CMV
  • Tags / Fusion Proteins
    • V5
    • HA

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer acgactcactatagggagaccc
  • 3′ sequencing primer GATCAGCGAGCTCTAGctcgag
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-CMV-CD4-sTC-M13-GFP-IRES-CaM-V5-sTN was a gift from Christina Kim (Addgene plasmid # 219782 ; http://n2t.net/addgene:219782 ; RRID:Addgene_219782)

  • For your References section:

    Rapid, biochemical tagging of cellular activity history in vivo. Zhang R, Anguiano M, Aarrestad IK, Lin S, Chandra J, Vadde SS, Olson DE, Kim CK. Nat Methods. 2024 Sep;21(9):1725-1735. doi: 10.1038/s41592-024-02375-7. Epub 2024 Aug 5. 10.1038/s41592-024-02375-7 PubMed 39103446
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