pAAV-CMV-CD4-sTC-M13-GFP-P2A-CaM-V5-sTN
(Plasmid #219781)

• Purpose: CaST-P2A for HEK cell expression
• Depositing Lab: Christina Kim
• Publication: Zhang et al Nat Methods. 2024 Sep;21(9):1725-1735. doi: 10.1038/s41592-024-02375-7. Epub 2024 Aug 5.

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Backbone

• Vector backbone: AAV
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: CD4-sTC-M13-GFP-P2A-CaM-V5-sTN
• Species: Synthetic
• Promoter: CMV
• Tags / Fusion Proteins:
  • V5
  • HA

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: acgactcactatagggagaccc
• 3′ sequencing primer: GATCAGCGAGCTCTAGctcgag

Resource Information

• Supplemental Documents:
  • CaST-P2A for HEK cell expression.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://www.biorxiv.org/content/10.1101/2023.09.06.556431v1 for bioRxiv preprint.