pSL1419
(Plasmid #219778)

• Purpose: pDEF-TurboID-GFPmut2, negative control for cytosolic expression
• Depositing Lab: Scott Lindner
• Publication: Rios et al bioRxiv [Preprint]. 2024 Mar 16:2024.02.01.577866. doi: 10.1101/2024.02.01.577866.

Backbone

• Vector backbone: pDEF
• Vector type: Plasmodium, rodent malaria parasites
• Selectable markers: Human DHFR

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: TurboID/GFPmut2
• Species: Synthetic
• Insert Size (bp): 1709
• Promoter: PbEF1alpha promoter

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: KpnI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: n/a

Resource Information

• Supplemental Documents:
  • pSL1419 (PSd).gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid can be integrated into the p230p locus for Plasmodium yoelii (and likely also Plasmodium berghei). The TurboID/GFPmut2 fusion protein is expressed via a PbEF1alpha promoter to create an unfused control protein that is expressed in a cytosolic diffuse manner. This is used as a negative control for TurboID proximity proteomics for proteins that are found in the cytosol.