pNK3071
(Plasmid
#219755)
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PurposeMoClo-compatible Level P vector for improved autonomous bioluminescence in plants encoding kanamycin resistance cassette, mcitHispS, NpgA, nnH3H_v2, nnLuz_v4 and nnCPH.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 219755 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneLevel P-like
- Backbone size w/o insert (bp) 4919
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Vector typePlant Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemcitHispS, NpgA, nnH3H_v2, nnLuz_v4 and nnCPH
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Alt nameFBP3
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SpeciesEscherichia coli, Neonothopanus nambi, Aspergillus nidulans, Mycena citricolor
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Insert Size (bp)17306
Cloning Information
- Cloning method Golden Gate
- 5′ sequencing primer ggcaggatatattgtggtgta
- 3′ sequencing primer ggataaaccttttcacgccct (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made bySynthetic
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
pNOS - KanR - tOCS | p35s_0.4kb - 5'UTR_TMV - mcitHispS - tOCS | pCmYLCV - NpgA - tATP | p35s_1.3kb - 5'UTR_TMV - nnLuz_v4 - tAct2 | p35s_0.4kb - 5'UTR_TMV - nnCPH - tOCS | pFMV - nnH3H_v2 - tNOS
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pNK3071 was a gift from Alexander Mishin (Addgene plasmid # 219755 ; http://n2t.net/addgene:219755 ; RRID:Addgene_219755) -
For your References section:
An improved pathway for autonomous bioluminescence imaging in eukaryotes. Shakhova ES, Karataeva TA, Markina NM, Mitiouchkina T, Palkina KA, Perfilov MM, Wood MG, Hoang TT, Hall MP, Fakhranurova LI, Alekberova AE, Malyshevskaia AK, Gorbachev DA, Bugaeva EN, Pletneva LK, Babenko VV, Boldyreva DI, Gorokhovatsky AY, Balakireva AV, Gao F, Choob VV, Encell LP, Wood KV, Yampolsky IV, Sarkisyan KS, Mishin AS. Nat Methods. 2024 Mar;21(3):406-410. doi: 10.1038/s41592-023-02152-y. Epub 2024 Jan 22. 10.1038/s41592-023-02152-y PubMed 38253843