L0I75_tUBQ10_GG5-GG6
(Plasmid
#219714)
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PurposeLevel 0 tUBQ10 terminator, GG5 and GG6 BsaI sites for golden gate assembly.
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 219714 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCR-BluntII-TOPO
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Backbone manufacturerThermo fisher Scientific
- Backbone size w/o insert (bp) 3512
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Vector typeSynthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGG5-tUBQ10-GG6
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SpeciesA. thaliana (mustard weed)
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Insert Size (bp)666
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MutationWT terminator with added golden gate linkers
Cloning Information
- Cloning method Golden Gate
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
L0I75_tUBQ10_GG5-GG6 was a gift from Jennifer Nemhauser (Addgene plasmid # 219714 ; http://n2t.net/addgene:219714 ; RRID:Addgene_219714)