L1PB26- pAHP6--PhiC31-NLS-tUBQ1 + pGATA23--Bxb1-SAUR-tUBQ10
(Plasmid
#219701)
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PurposeLevel 1 PhiC31 and Bxb1 integrase construct for tracking of lateral root development: pAHP6 promoter for PhiC31 and pGATA23 promoter for Bxb1, NLS tag for PhiC31 and RNA degradation tag for Bxb1
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 219701 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepGII-L1-Rep
- Backbone size w/o insert (bp) 4708
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Vector typePlant Expression
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namepAHP6::PhiC31-NLS:tUBQ1 + pGATA23::Bxb1-SAUR:tUBQ10
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SpeciesSynthetic
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Insert Size (bp)8341
Cloning Information
- Cloning method Golden Gate
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
L1PB26- pAHP6--PhiC31-NLS-tUBQ1 + pGATA23--Bxb1-SAUR-tUBQ10 was a gift from Jennifer Nemhauser (Addgene plasmid # 219701 ; http://n2t.net/addgene:219701 ; RRID:Addgene_219701)