L1T10 - T2PB/pUBQ10 (mTagBFP2, mCherry, NeonGreen)
(Plasmid
#219687)
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PurposeLevel 1 History-dependent target, switch PhiC31 then Bxb1, pUBQ10 promoter, and output: mtagBFP2 then mCherry then NeonGreen (for transformation, Kan plant resistance, Kan bacteria resistance).
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 219687 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepGII-L1-Rep (Kan)
- Backbone size w/o insert (bp) 6333
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Vector typePlant Expression
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Selectable markersKanamycin
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameT2PB/pUBQ10 (mTagBFP2, mCherry, NeonGreen)
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SpeciesSynthetic
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Insert Size (bp)6420
Cloning Information
- Cloning method Golden Gate
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
L1T10 - T2PB/pUBQ10 (mTagBFP2, mCherry, NeonGreen) was a gift from Jennifer Nemhauser (Addgene plasmid # 219687 ; http://n2t.net/addgene:219687 ; RRID:Addgene_219687)