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Addgene

Strep-Halo
(Plasmid #219682)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 219682 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCDNA3.1
  • Backbone size w/o insert (bp) 5376
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    StrepTag-HaloTag
  • Species
    Synthetic
  • Insert Size (bp)
    1014
  • Promoter CMV & T7

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Thermo

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2024.12.20.629623 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Strep-Halo was a gift from Alison Twelvetrees (Addgene plasmid # 219682 ; http://n2t.net/addgene:219682 ; RRID:Addgene_219682)

  • For your References section:

    Kinesin-1 is highly flexible and adopts an open conformation in the absence of cargo. Smith ER, Turner ED, Abdelhamid MAS, Craggs TD, Twelvetrees AE. bioRxiv 2024.12.20.629623 10.1101/2024.12.20.629623
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