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Addgene

K560-Halo-Strep
(Plasmid #219681)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 219681 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMV
  • Backbone size w/o insert (bp) 1479
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    KIF5B
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2595
  • Mutation
    1-560
  • Entrez Gene
    KIF5B (a.k.a. HEL-S-61, KINH, KNS, KNS1, UKHC)
  • Promoter CMV & T7
  • Tag / Fusion Protein
    • HaloTag, Strep tag (C terminal on insert)

Cloning Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2024.12.20.629623 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    K560-Halo-Strep was a gift from Alison Twelvetrees (Addgene plasmid # 219681 ; http://n2t.net/addgene:219681 ; RRID:Addgene_219681)

  • For your References section:

    Kinesin-1 is highly flexible and adopts an open conformation in the absence of cargo. Smith ER, Turner ED, Abdelhamid MAS, Craggs TD, Twelvetrees AE. bioRxiv 2024.12.20.629623 10.1101/2024.12.20.629623
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