CROPseq-3P5P
(Plasmid
#219680)
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Purpose(Empty Backbone) CROPseq vector enabling gRNA detection via mRNA-based capture (CROPseq), 3'-based direct capture using 10x capture sequence 1 or 5'-based enrichment using a custom reverse transcription primer
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 219680 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneCROPseq-Guide-Puro-mCherry2 (#219679)
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Backbone manufacturerEmpty Backbone
- Backbone size (bp) 11017
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Modifications to backboneModified gRNA backbone including 10x Capture Sequence 1, added primer binding site
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Vector typeMammalian Expression, Lentiviral, CRISPR
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Selectable markersPuromycin ; mCherry2
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Tag
/ Fusion Protein
- EF1a-Puro-P2A-mCherry2-WPRE-hU6-gRNA
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
CROPseq-3P5P was a gift from Christoph Bock (Addgene plasmid # 219680 ; http://n2t.net/addgene:219680 ; RRID:Addgene_219680)
Map uploaded by the depositor.
