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CROPseq-Guide-Puro-mCherry2
(Plasmid #219679)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 219679 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    CROPseq-Guide-Puro (#86708)
  • Backbone manufacturer
    Christoph Bock Lab
  • Backbone size (bp) 10988
  • Modifications to backbone
    Added P2A-mCherry2
  • Vector type
    Mammalian Expression, Lentiviral, CRISPR
  • Selectable markers
    Puromycin ; mCherry2
  • Tag / Fusion Protein
    • EF1a-Puro-P2A-mCherry2-WPRE-hU6-gRNA

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CROPseq-Guide-Puro-mCherry2 was a gift from Christoph Bock (Addgene plasmid # 219679 ; http://n2t.net/addgene:219679 ; RRID:Addgene_219679)

  • For your References section:

    Stress signaling boosts interferon-induced gene transcription in macrophages. Boccuni L, Podgorschek E, Schmiedeberg M, Platanitis E, Traxler P, Fischer P, Schirripa A, Novoszel P, Nebreda AR, Arthur JSC, Fortelny N, Farlik M, Sexl V, Bock C, Sibilia M, Kovarik P, Muller M, Decker T. Sci Signal. 2022 Dec 13;15(764):eabq5389. doi: 10.1126/scisignal.abq5389. Epub 2022 Dec 13. 10.1126/scisignal.abq5389 PubMed 36512641
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