Skip to main content
Addgene

pRER-PhoCl-Insert
(Plasmid #219675)

Print

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 219675 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
Add to Cart

* Log in to view industry pricing.

Backbone

  • Vector backbone
    pPRETight2
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    RER-PhoCl
  • Promoter TRE
  • Tag / Fusion Protein
    • PhoCl

Cloning Information

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

All the plasmids with pTRE vectors have the POI under tetracycline
promoter and hence when transfected needs to be transfected with another
plasmid, rtTA3(Sara knows) in the presence of doxycycline. or it can be
transfected in cells stably expressing the tetracycline-on transactivator
(rtTA3). rtTA3 is the transactivator that binds with doxycycline and sits
at the promoter for initiation of transcription.

While cloning please make sure you include signal peptide in the beginning
if needed. This depends on the specific POI.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRER-PhoCl-Insert was a gift from Helge Ewers (Addgene plasmid # 219675 ; http://n2t.net/addgene:219675 ; RRID:Addgene_219675)

  • For your References section:

    An optogenetic method for the controlled release of single molecules. Kashyap P, Bertelli S, Cao F, Kostritskaia Y, Blank F, Srikanth NA, Schlack-Leigers C, Saleppico R, Bierhuizen D, Lu X, Nickel W, Campbell RE, Plested AJR, Stauber T, Taylor MJ, Ewers H. Nat Methods. 2024 Mar 8. doi: 10.1038/s41592-024-02204-x. 10.1038/s41592-024-02204-x PubMed 38459384
Related items: