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Addgene

pTMEM-PhoCl-Insert
(Plasmid #219674)

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Item Catalog # Description Quantity Price (USD)
Plasmid 219674 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pCMVHygro
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    TMEM-PhoCl-rtTA3
  • Promoter CMV
  • Tag / Fusion Protein
    • PhoCl

Cloning Information

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTMEM-PhoCl-Insert was a gift from Helge Ewers (Addgene plasmid # 219674 ; http://n2t.net/addgene:219674 ; RRID:Addgene_219674)

  • For your References section:

    An optogenetic method for the controlled release of single molecules. Kashyap P, Bertelli S, Cao F, Kostritskaia Y, Blank F, Srikanth NA, Schlack-Leigers C, Saleppico R, Bierhuizen D, Lu X, Nickel W, Campbell RE, Plested AJR, Stauber T, Taylor MJ, Ewers H. Nat Methods. 2024 Mar 8. doi: 10.1038/s41592-024-02204-x. 10.1038/s41592-024-02204-x PubMed 38459384
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