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Addgene

pUASTattB-dALFANb(5mut)-mGreenLantern
(Plasmid #219661)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 219661 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUASTattB
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Destabilised ALFA nanobody (5 mutations) fused to mGreenLantern
  • Insert Size (bp)
    1120

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2022.06.17.496589 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUASTattB-dALFANb(5mut)-mGreenLantern was a gift from Markus Affolter (Addgene plasmid # 219661 ; http://n2t.net/addgene:219661 ; RRID:Addgene_219661)

  • For your References section:

    Seamless knockins in Drosophila via CRISPR-triggered single-strand annealing. Aguilar G, Bauer M, Vigano MA, Schnider ST, Brugger L, Jimenez-Jimenez C, Guerrero I, Affolter M. Dev Cell. 2024 Oct 7;59(19):2672-2686.e5. doi: 10.1016/j.devcel.2024.06.004. Epub 2024 Jul 5. 10.1016/j.devcel.2024.06.004 PubMed 38971155
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