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Addgene

pSEED
(Plasmid #219658)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 219658 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUC19-Kan

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Empty SEED Cassette (3xP3-dsRed)
  • Insert Size (bp)
    1300

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2022.06.17.496589 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSEED was a gift from Markus Affolter (Addgene plasmid # 219658 ; http://n2t.net/addgene:219658 ; RRID:Addgene_219658)

  • For your References section:

    Seamless knockins in Drosophila via CRISPR-triggered single-strand annealing. Aguilar G, Bauer M, Vigano MA, Schnider ST, Brugger L, Jimenez-Jimenez C, Guerrero I, Affolter M. Dev Cell. 2024 Oct 7;59(19):2672-2686.e5. doi: 10.1016/j.devcel.2024.06.004. Epub 2024 Jul 5. 10.1016/j.devcel.2024.06.004 PubMed 38971155
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