OgeuIscB ωRNA-v13
(Plasmid
#219634)
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Purposehuman U6-driven expression of OgeuIscB omegaRNA-v13 (ωRNA-v13) scaffold with BsmBI for guide cloning
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 219634 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBR322
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Vector typeMammalian Expression, CRISPR
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameOgeuIscB-ωRNA-v13 scaffold with BsmBI for guide cloning
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gRNA/shRNA sequenceagagacgAAAAGAGTGAACGAGAtcgtctca
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
OgeuIscB ωRNA-v13 was a gift from Weixin Tang (Addgene plasmid # 219634 ; http://n2t.net/addgene:219634 ; RRID:Addgene_219634) -
For your References section:
Assessing and engineering the IscB-omegaRNA system for programmed genome editing. Yan H, Tan X, Zou S, Sun Y, Ke A, Tang W. Nat Chem Biol. 2024 Jul 8. doi: 10.1038/s41589-024-01669-3. 10.1038/s41589-024-01669-3 PubMed 38977787