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Addgene

pCDSRaD-sdi1
(Plasmid #219566)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 219566 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCDSRaD
  • Backbone size w/o insert (bp) 3400
  • Total vector size (bp) 5499
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    sdi1
  • Alt name
    p21 CDK inhibitor
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2100
  • Entrez Gene
    CDKN1A (a.k.a. CAP20, CDKN1, CIP1, MDA-6, P21, SDI1, WAF1, p21CIP1)
  • Promoter SRa

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamH1 (not destroyed)
  • 3′ cloning site BamH1 (not destroyed)
  • 5′ sequencing primer GTCCCGGATCCGGTGGTGG
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCDSRaD-sdi1 was a gift from Asao Noda & James R Smith (Addgene plasmid # 219566 ; http://n2t.net/addgene:219566 ; RRID:Addgene_219566)

  • For your References section:

    Cloning of senescent cell-derived inhibitors of DNA synthesis using an expression screen. Noda A, Ning Y, Venable SF, Pereira-Smith OM, Smith JR. Exp Cell Res. 1994 Mar;211(1):90-8. doi: 10.1006/excr.1994.1063. 10.1006/excr.1994.1063 PubMed 8125163
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