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Addgene

pAAV-G12-hFGF1
(Plasmid #219554)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 219554 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pG12
  • Backbone manufacturer
    GeneScript
  • Backbone size w/o insert (bp) 3647
  • Vector type
    Mammalian Expression, AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    hFGF1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    475
  • Entrez Gene
    FGF1 (a.k.a. AFGF, ECGF, ECGF-beta, ECGFA, ECGFB, FGF-1, FGF-alpha, FGFA, GLIO703, HBGF-1, HBGF1)
  • Promoter 12xUAS with TATA-box minimal promoter

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AgeI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer pBR322ori-F
  • 3′ sequencing primer WPRE-R
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

To make pAAV-G12-hFGF1, hFGF1 was amplified from pDONR223_FGF1_WT, a gift from Jesse Boehm, William Hahn, and David Root (Addgene plasmid #81825). Subsequently, the amplified hFGF1 was swapped with AkaLuc in pAAV-G12-AkaLuc using AgeI and EcoRI restriction enzymes.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-G12-hFGF1 was a gift from Vladislav Verkhusha (Addgene plasmid # 219554 ; http://n2t.net/addgene:219554 ; RRID:Addgene_219554)

  • For your References section:

    iLight2: A near-infrared optogenetic tool for gene transcription with low background activation. Baloban M, Kasatkina LA, Verkhusha VV. Protein Sci. 2024 May;33(5):e4993. doi: 10.1002/pro.4993. 10.1002/pro.4993 PubMed 38647395
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