pAAV-G12-mFGF2
(Plasmid #219552)

• Purpose: mFGF2 expression in mammlian cells. pAAV vector. Reporter plasmid.
• Depositing Lab: Vladislav Verkhusha
• Publication: Baloban et al Protein Sci. 2024 May;33(5):e4993. doi: 10.1002/pro.4993.

Backbone

• Vector backbone: pG12
• Backbone manufacturer: GeneScript
• Backbone size w/o insert (bp): 3630
• Vector type: Mammalian Expression, AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: mFGF2
• Species: M. musculus (mouse)
• Insert Size (bp): 484
• Entrez Gene: Fgf2 (a.k.a. Fgf-2, Fgf2a, Fgfb, bFGF)
• Promoter: 12xUAS with TATA-box minimal promoter

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: pBR322ori-F
• 3′ sequencing primer: WPRE-R

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

To make pAAV-G12-mFGF2, mFGF2 was amplified from Addgene plasmid #22084, a gift from Gail Martin. Then, the amplified mFGF2 was swapped with AkaLuc in pAAV-G12-AkaLuc using BamHI and EcoRI restriction enzymes, with a partial digest being used in the case of mFGF2 with the BamHI enzyme.