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Addgene

pAAV-G12-AkaLuc
(Plasmid #219551)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 219551 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pG12
  • Backbone manufacturer
    GeneScript
  • Backbone size w/o insert (bp) 3635
  • Vector type
    Mammalian Expression, AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    AkaLuc
  • Species
    Synthetic
  • Insert Size (bp)
    1672
  • GenBank ID
    LC320664.1
  • Promoter 12xUAS with TATA-box minimal promoter

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer pBR322ori-F
  • 3′ sequencing primer WPRE-R
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

To make a pAAV-G12-AkaLuc plasmid, the fragment encoding AkaLuc was amplified from pAAV-TRE-Tight2-AkaLuc (Addgene #186186) and swapped with CheRiff-T2AmCherry in pAAV-G12-CheRiff-T2A-mCherry (Addgene #170275) using BamHI and EcoRI restriction sites.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-G12-AkaLuc was a gift from Vladislav Verkhusha (Addgene plasmid # 219551 ; http://n2t.net/addgene:219551 ; RRID:Addgene_219551)

  • For your References section:

    iLight2: A near-infrared optogenetic tool for gene transcription with low background activation. Baloban M, Kasatkina LA, Verkhusha VV. Protein Sci. 2024 May;33(5):e4993. doi: 10.1002/pro.4993. 10.1002/pro.4993 PubMed 38647395
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