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Addgene

pAAV-CMVd1-NLS-GAL4-iLight-VP16
(Plasmid #219549)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 219549 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAAV-CW3SL-EGFP
  • Backbone manufacturer
    Addgene #61463
  • Backbone size w/o insert (bp) 3514
  • Vector type
    Mammalian Expression, AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NLS-Gal4(1-65)-iLight-VP16
  • Species
    Synthetic
  • Insert Size (bp)
    2382
  • GenBank ID
    MW890755.1 (part of the insert)
  • Promoter CMVd1

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer pBR322ori-F
  • 3′ sequencing primer WPRE-R
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

To make pAAV-CMVd1-NLS-GAL4(1-65)-iLight-VP16, an additional NheI restriction site was introduced upstream of the CaMKII promoter in the partiall modified pAAV-CaMKII-GAL4-iLight-VP16 plasmid (Addgene #170274). Subsequently, the CaMKII promoter was replaced with CMVd1 using NheI restriction sites.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-CMVd1-NLS-GAL4-iLight-VP16 was a gift from Vladislav Verkhusha (Addgene plasmid # 219549 ; http://n2t.net/addgene:219549 ; RRID:Addgene_219549)

  • For your References section:

    iLight2: A near-infrared optogenetic tool for gene transcription with low background activation. Baloban M, Kasatkina LA, Verkhusha VV. Protein Sci. 2024 May;33(5):e4993. doi: 10.1002/pro.4993. 10.1002/pro.4993 PubMed 38647395
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