pAAV-CMVd1-NLS-GAL4-iLight-VP16
(Plasmid #219549)

• Purpose: NLS-Gal4(1-65)-iLight-VP16 construc expression in mammalian cells. pAAV vector.
• Depositing Lab: Vladislav Verkhusha
• Publication: Baloban et al Protein Sci. 2024 May;33(5):e4993. doi: 10.1002/pro.4993.

Backbone

• Vector backbone: pAAV-CW3SL-EGFP
• Backbone manufacturer: Addgene #61463
• Backbone size w/o insert (bp): 3514
• Vector type: Mammalian Expression, AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: NLS-Gal4(1-65)-iLight-VP16
• Species: Synthetic
• Insert Size (bp): 2382
• GenBank ID: MW890755.1 (part of the insert)
• Promoter: CMVd1

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: pBR322ori-F
• 3′ sequencing primer: WPRE-R

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

To make pAAV-CMVd1-NLS-GAL4(1-65)-iLight-VP16, an additional NheI restriction site was introduced upstream of the CaMKII promoter in the partiall modified pAAV-CaMKII-GAL4-iLight-VP16 plasmid (Addgene #170274). Subsequently, the CaMKII promoter was replaced with CMVd1 using NheI restriction sites.