hfDECOR-F148A
(Plasmid
#219546)
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PurposeExpression of CMV-TadA8e-F148A-NLS-dCas13d-NLS in mammalian cells (hfDECOR-F148A expression)
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 219546 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCMV with a BR322 origin
- Total vector size (bp) 6999
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Vector typeMammalian Expression, CRISPR
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namehfDECOR-F148A
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Alt nameTadA8e-F148A-dCas13d
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SpeciesSynthetic
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Insert Size (bp)3597
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MutationChanged TadA8e Phenylalanine 148 to Alanine
- Promoter CMV
Cloning Information
- Cloning method Other
- 5′ sequencing primer CMV Forward
- 3′ sequencing primer BGH Reverse (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
hfDECOR-F148A was a gift from Weixin Tang (Addgene plasmid # 219546 ; http://n2t.net/addgene:219546 ; RRID:Addgene_219546) -
For your References section:
Programmed RNA editing with an evolved bacterial adenosine deaminase. Yan H, Tang W. Nat Chem Biol. 2024 Jul 5. doi: 10.1038/s41589-024-01661-x. 10.1038/s41589-024-01661-x PubMed 38969862
Map uploaded by the depositor.
