pLenti-Cre-MS2_UbC-NLS-tdMCP-GFP (Cre_tdMCP-GFP)
(Plasmid #219537)

• Purpose: a. Expresses Cre recombinase tagged with 23x repeats of MS2 binding sequence under EF1A promotor and b. NLS-tdMCP_GFP reporter under UbC promotor
• Depositing Lab: Jeffrey Gerst
• Publication: Haimovich et al bioRxiv 2024.11.06.622258

Backbone

• Vector backbone: pRRLSIN.cPPT.PGK-GFP.WPRE
• Vector type: Mammalian Expression, Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Growth instructions: NEB Stable is preferred. Although stbl3 can be used. The plasmid is prone to recombination due to MS2 repeats.
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: Cre recombinase
• Alt name: Cre
• Insert Size (bp): 1029
• Entrez Gene: cre (a.k.a. P1_gp003)

Gene/Insert 2

• Gene/Insert name: NLS-tdMCP-GFP
• Alt name: MS2 coat protein tagged with GFP

Resource Information

• Supplemental Documents:
  • pLenti-Cre-MS2_UbC-NLS-tdMCP-GFP (Cre_tdMCP-GFP)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Industry MTA

Depositor Comments

1. A restriction-based cloning method is highly recommended for any changes/manipulation of this plasmid. Amplification of this plasmid OR restriction-free cloning enzymes may lead to undesired recombination.

2. The inserts "EF1A-Cre-MS2-RBGpA" and "UbC-NLS-tdMCP-GFP-pA" are placed in opposite orientations to ensure efficient lentivirus packaging. Further design consideration is based on https://www.intechopen.com/chapters/16787.

Please visit https://doi.org/10.1101/2024.11.06.622258 for bioRxiv preprint.