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pLenti-Cre-MS2_UbC-NLS-tdMCP-GFP (Cre_tdMCP-GFP)
(Plasmid #219537)

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Item Catalog # Description Quantity Price (USD)
Plasmid 219537 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pRRLSIN.cPPT.PGK-GFP.WPRE
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Growth instructions
    NEB Stable is preferred. Although stbl3 can be used. The plasmid is prone to recombination due to MS2 repeats.
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    Cre recombinase
  • Alt name
    Cre
  • Insert Size (bp)
    1029
  • Entrez Gene
    cre (a.k.a. P1_gp003)

Gene/Insert 2

  • Gene/Insert name
    NLS-tdMCP-GFP
  • Alt name
    MS2 coat protein tagged with GFP

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

1. A restriction-based cloning method is highly recommended for any changes/manipulation of this plasmid. Amplification of this plasmid OR restriction-free cloning enzymes may lead to undesired recombination.

2. The inserts "EF1A-Cre-MS2-RBGpA" and "UbC-NLS-tdMCP-GFP-pA" are placed in opposite orientations to ensure efficient lentivirus packaging. Further design consideration is based on https://www.intechopen.com/chapters/16787.

Please visit https://doi.org/10.1101/2024.11.06.622258 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLenti-Cre-MS2_UbC-NLS-tdMCP-GFP (Cre_tdMCP-GFP) was a gift from Jeffrey Gerst (Addgene plasmid # 219537 ; http://n2t.net/addgene:219537 ; RRID:Addgene_219537)

  • For your References section:

    Complementation of a human disease phenotype in vitro by intercellular mRNA transfer. Haimovich G, Dasgupta S, Ravi AG, Gerst JE. bioRxiv 2024.11.06.622258 10.1101/2024.11.06.622258
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